QUANTITATIVE-ANALYSIS OF CADHERIN-CATENIN-ACTIN REORGANIZATION DURINGDEVELOPMENT OF CELL-CELL ADHESION

Citation
Cl. Adams et al., QUANTITATIVE-ANALYSIS OF CADHERIN-CATENIN-ACTIN REORGANIZATION DURINGDEVELOPMENT OF CELL-CELL ADHESION, The Journal of cell biology, 135(6), 1996, pp. 1899-1911
Citations number
50
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell Biology
Journal title
ISSN journal
0021-9525
Volume
135
Issue
6
Year of publication
1996
Part
2
Pages
1899 - 1911
Database
ISI
SICI code
0021-9525(1996)135:6<1899:QOCRD>2.0.ZU;2-J
Abstract
Epithelial cell-cell adhesion requires interactions between opposing e xtracellular domains of E-cadherin, and among the cytoplasmic domain o f E-cadherin, catenins, and actin cytoskeleton. Little is known about how the cadherin-catenin-actin complex is assembled upon cell-cell con tact, or how these complexes initiate and strengthen adhesion. We have used time-lapse differential interference contrast (DIG) imaging to o bserve the development of cell-cell contacts, and quantitative retrosp ective immunocytochemistry to measure recruitment of proteins to those contacts. We show that E-cadherin, alpha-catenin, and beta-catenin, b ut not plakoglobin, coassemble into Triton X-100 insoluble (TX-insolub le) structures at cell-cell contacts with kinetics similar to those fo r strengthening of E-cadherin-mediated cell adhesion (Angres, B., A. E arth, and W.J. Nelson. 1996. J. Cell Biol. 134:549-557). TX-insoluble E-cadherin, alpha-catenin, and beta-catenin colocalize along cell-cell contacts in spatially discrete micro-domains which we designate ''pun cta,'' and the relative amounts of each protein in each punctum increa se proportionally, As the length of the contact increases, the number of puncta increases proportionally along the contact and each punctum is associated with a bundle of actin filaments. These results indicate that localized clustering of E-cadherin/catenin complexes into puncta and their association with actin is involved in initiating cell conta cts, Subsequently, the spatial ordering of additional puncta along the contact may be involved in zippering membranes together, resulting in rapid strengthening of adhesion.