CONTROL OF THE LIMB BUD OUTGROWTH IN QUAIL-CHICK CHIMERA

Citation
H. Ohkihamazaki et al., CONTROL OF THE LIMB BUD OUTGROWTH IN QUAIL-CHICK CHIMERA, Developmental dynamics, 208(1), 1997, pp. 85-91
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Developmental Biology","Anatomy & Morphology
Journal title
ISSN journal
1058-8388
Volume
208
Issue
1
Year of publication
1997
Pages
85 - 91
Database
ISI
SICI code
1058-8388(1997)208:1<85:COTLBO>2.0.ZU;2-0
Abstract
It is well known that the apical ectodermal ridge (AER) and the zone o f polarizing activity (ZPA) play a major role in growth and patterning of the limb. But a mechanism underlying species-specific growth of th e limb has not yet been fully elucidated. To investigate the role of A ER and ZPA in limb size control, we constructed quail-chick limb chime ras. When we grafted a whole forelimb bud from one species to another, the size of the developed grafted limb was comparable to the limb of the donor species, Moreover, we demonstrated that neither the interspe cific substitution of the posterior half region of the limb bud contai ning the ZPA nor the exchange of the ectodermal component of the limb involving the AER could alter the species-specific size of the limb. T hese results indicate that the factors affecting the size of the limb are already involved in the mesodermal component of the limb bud at st age 20 of chick embryo. Thus, the mesoderm dictates limb specificity i ncluding size. (C) 1997 Wiley-Liss, Inc.