METHYLPREDNISOLONE ATTENUATES AIRWAY AND VASCULAR-RESPONSES INDUCED BY REACTIVE OXYGEN SPECIES IN ISOLATED, PLASMA-PERFUSED RAT LUNGS

Citation
J. Kjaeve et al., METHYLPREDNISOLONE ATTENUATES AIRWAY AND VASCULAR-RESPONSES INDUCED BY REACTIVE OXYGEN SPECIES IN ISOLATED, PLASMA-PERFUSED RAT LUNGS, Free radical research, 25(5), 1996, pp. 407-414
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology
Journal title
ISSN journal
1071-5762
Volume
25
Issue
5
Year of publication
1996
Pages
407 - 414
Database
ISI
SICI code
1071-5762(1996)25:5<407:MAAAVI>2.0.ZU;2-C
Abstract
The effects of methylprednisolone (MP) on the acute airway and pulmona ry vascular responses induced by reactive oxygen species (ROS) were in vestigated in isolated, plasma-perfused rat lungs. ROS were generated by adding xanthine oxidase and hypoxanthine to the perfusate. MP was a dministered in 3 different ways: 1. Added to the perfusate (1 mgml(-1 )) 5 min prior to xanthine oxidase and hypoxanthine, 2. Given as intra peritoneal injections (40 mgkg(-1)) to lung donor rats 12 and 2 hours prior to the experiments, or 3. Combining 1 and 2. The lungs were per fused at constant volume inflow (15 mlmin(-1)). Pulmonary arterial pr essure and transpulmonary pressure were followed for 30 min after addi tion of xanthine oxidase and hypoxanthine. ROS induced a powerful, acu te broncho- and vasoconstriction, which was inhibited by addition of M P to the perfusate. Pretreatment with MP also inhibited the vascular a nd airway responses. Adding MP to the perfusate of pretreated lungs fu rther reduced the ROS-induced smooth muscle constriction. In conclusio n, MP inhibits vasoconstriction and bronchoconstriction induced by ROS in isolated rat lungs.