S. Kobayashi et al., INHIBITORY EFFECT OF MAGNOSALIN DERIVED FROM FLOS-MAGNOLIAE ON TUBE FORMATION OF RAT VASCULAR ENDOTHELIAL-CELLS DURING THE ANGIOGENIC PROCESS, Biological & pharmaceutical bulletin, 19(10), 1996, pp. 1304-1306
An aqueous water extract of Flos magnoliae, a Japanese Sine-medicine,
inhibits angiogenesis in adjuvant-induced mouse pouch granuloma, Magno
salin (MSA) and magnoshinin (MSI), neolignans isolated from magnolia,
have a crucial role in the anti-angiogenic effect of magnolia (Kimura
et al., Int, Arch. Allergy Appl. Immunol., 93, 365 (1990); Phytother.
Res., 6, 209 (1992)), We investigated the effects of these neolignans
on tube formation of endothelial cells (EC) cultured in type I collage
n gel during the angiogenic process. MSA (0.1-10 mu M), MSI (0.23-7 mu
M) and corticosterone (CS: 0.3-30 mu M) inhibited fetal bovine serum
(FBS)-stimulated tube formation in a concentration-dependent manner. T
heir 30% inhibitory concentration (IC30, 95% confidence limits) values
were 0.51 (0.20-1.27) for MSA, 8.14 (2.48-26.7) for MSI and 3.65 mu M
(2.47-5.40) for CS, respectively, MSA and MSI (1-3 mu M) also inhibit
ed interleukin (IL)-1 alpha-stimulated tube formation in a concentrati
on-dependent manner. Their IC50 values (95% confidence limits) were 1.
22 (1.01-1.47) for MSA and 0.74 mu M (0.24-2.31) for MSI against a sub
maximal concentration (69 pM) of IL-1 alpha-stimulated tube formation.
Their inhibitory effects on the action of IL-1 alpha were non-competi
tive. These results demonstrate that MSA inhibited FBS-stimulated tube
formation with a greater potency than MSI. The inhibitory effect of M
SA on the action of FBS differed from that on the action of IL-1 alpha