INHIBITORY EFFECT OF MAGNOSALIN DERIVED FROM FLOS-MAGNOLIAE ON TUBE FORMATION OF RAT VASCULAR ENDOTHELIAL-CELLS DURING THE ANGIOGENIC PROCESS

Citation
S. Kobayashi et al., INHIBITORY EFFECT OF MAGNOSALIN DERIVED FROM FLOS-MAGNOLIAE ON TUBE FORMATION OF RAT VASCULAR ENDOTHELIAL-CELLS DURING THE ANGIOGENIC PROCESS, Biological & pharmaceutical bulletin, 19(10), 1996, pp. 1304-1306
Citations number
12
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Pharmacy
ISSN journal
0918-6158
Volume
19
Issue
10
Year of publication
1996
Pages
1304 - 1306
Database
ISI
SICI code
0918-6158(1996)19:10<1304:IEOMDF>2.0.ZU;2-G
Abstract
An aqueous water extract of Flos magnoliae, a Japanese Sine-medicine, inhibits angiogenesis in adjuvant-induced mouse pouch granuloma, Magno salin (MSA) and magnoshinin (MSI), neolignans isolated from magnolia, have a crucial role in the anti-angiogenic effect of magnolia (Kimura et al., Int, Arch. Allergy Appl. Immunol., 93, 365 (1990); Phytother. Res., 6, 209 (1992)), We investigated the effects of these neolignans on tube formation of endothelial cells (EC) cultured in type I collage n gel during the angiogenic process. MSA (0.1-10 mu M), MSI (0.23-7 mu M) and corticosterone (CS: 0.3-30 mu M) inhibited fetal bovine serum (FBS)-stimulated tube formation in a concentration-dependent manner. T heir 30% inhibitory concentration (IC30, 95% confidence limits) values were 0.51 (0.20-1.27) for MSA, 8.14 (2.48-26.7) for MSI and 3.65 mu M (2.47-5.40) for CS, respectively, MSA and MSI (1-3 mu M) also inhibit ed interleukin (IL)-1 alpha-stimulated tube formation in a concentrati on-dependent manner. Their IC50 values (95% confidence limits) were 1. 22 (1.01-1.47) for MSA and 0.74 mu M (0.24-2.31) for MSI against a sub maximal concentration (69 pM) of IL-1 alpha-stimulated tube formation. Their inhibitory effects on the action of IL-1 alpha were non-competi tive. These results demonstrate that MSA inhibited FBS-stimulated tube formation with a greater potency than MSI. The inhibitory effect of M SA on the action of FBS differed from that on the action of IL-1 alpha .