A FRAGMENT OF STAPHYLOCOCCAL NUCLEASE WITH AN OB-FOLD STRUCTURE SHOWSHYDROGEN-EXCHANGE PROTECTION FACTORS IN THE RANGE REPORTED FOR MOLTENGLOBULES

Citation
At. Alexandrescu et al., A FRAGMENT OF STAPHYLOCOCCAL NUCLEASE WITH AN OB-FOLD STRUCTURE SHOWSHYDROGEN-EXCHANGE PROTECTION FACTORS IN THE RANGE REPORTED FOR MOLTENGLOBULES, Protein science, 5(9), 1996, pp. 1942-1946
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology
Journal title
ISSN journal
0961-8368
Volume
5
Issue
9
Year of publication
1996
Pages
1942 - 1946
Database
ISI
SICI code
0961-8368(1996)5:9<1942:AFOSNW>2.0.ZU;2-B
Abstract
Hydrogen-exchange rates for an OB-fold subdomain fragment of staphyloc occal nuclease have been measured at pH 4.7 and 4 degrees C, condition s close to the minimum of acid/base catalyzed exchange. The strongest protection from solvent exchange is observed for residues from a five- stranded beta-barrel in the NMR structure of the protein. Protection f actors, calculated from the experimental hydrogen-exchange rates, rang e between 1 and 190. Similarly small protection factors have in many c ases been attributed to ''molten globule'' conformations that are supp osed to lack a specific tertiary structure. The present results sugges t that marginal protection from solvent exchange does not exclude well -defined structure.