EFFECT OF PROTECTANTS IN L-DRYING ON THE CONFORMATION AND INFECTIVITYOF RICE DWARF PHYTOREOVIRUS

Citation
F. Fukumoto et al., EFFECT OF PROTECTANTS IN L-DRYING ON THE CONFORMATION AND INFECTIVITYOF RICE DWARF PHYTOREOVIRUS, Archives of virology, 141(8), 1996, pp. 1579-1585
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Virology
Journal title
ISSN journal
0304-8608
Volume
141
Issue
8
Year of publication
1996
Pages
1579 - 1585
Database
ISI
SICI code
0304-8608(1996)141:8<1579:EOPILO>2.0.ZU;2-O
Abstract
Purified rice dwarf phytoreovirus preparations, after rehydration foll owing drying without freezing (L-drying) and sucrose density gradient centrifugation, sedimented to the same position as untreated controls. Upon storage at 65 degrees C, virion conformation in L-dried preparat ions supplemented with 1% sucrose was maintained better than without a dditives. Moreover, during storage for 6 years at -70 degrees C, infec tivity of L-dried preparations from crude extracts of infected rice pl ants containing 5% sucrose was higher than controls based on the numbe r of count of infected foci on cell monolayers and transmission to ric e seedlings by leafhopper of the vector Nephotettix cincticeps, which had been injected with such extracts.