3-DIMENSIONAL RECONSTRUCTION OF PAINTED HUMAN INTERPHASE CHROMOSOMES - ACTIVE AND INACTIVE X-CHROMOSOME TERRITORIES HAVE SIMILAR VOLUMES BUT DIFFER IN SHAPE AND SURFACE-STRUCTURE

Citation
R. Eils et al., 3-DIMENSIONAL RECONSTRUCTION OF PAINTED HUMAN INTERPHASE CHROMOSOMES - ACTIVE AND INACTIVE X-CHROMOSOME TERRITORIES HAVE SIMILAR VOLUMES BUT DIFFER IN SHAPE AND SURFACE-STRUCTURE, The Journal of cell biology, 135(6), 1996, pp. 1427-1440
Citations number
53
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell Biology
Journal title
ISSN journal
0021-9525
Volume
135
Issue
6
Year of publication
1996
Part
1
Pages
1427 - 1440
Database
ISI
SICI code
0021-9525(1996)135:6<1427:3ROPHI>2.0.ZU;2-F
Abstract
This study provides a three-dimensional (3D) analysis of differences b etween the 3D morphology of active and inactive human X interphase chr omosomes (Xa and Xi territories), Chromosome territories were painted in formaldehyde-fixed, three-dimensionally intact human diploid female amniotic fluid cell nuclei (46, XX) with X-specific whole chromosome composite probes, The colocalization of a 4,6-diamidino-2-phenylindole dihydrochloride-stained Barr body with one of the two painted X terri tories allowed the unequivocal discrimination of the inactive X from i ts active counterpart, Light optical serial sections were obtained wit h a confocal laser scanning microscope. 3D-reconstructed Xa territorie s revealed a flatter shape and exhibited a larger and more irregular s urface when compared to the apparently smoother surface and rounder sh ape of Xi territories. The relationship between territory surface and volume was quantified by the determination of a dimensionless roundnes s factor (RF), RF and surface area measurements showed a highly signif icant difference between Xa and Xi territories (P < 0.001) in contrast to volume differences(P > 0.1), For comparison with an autosome of si milar DNA content, chromosome 7 territories were additionally painted. The 3D morphology of the two chromosome 7 territories was similar to the Xa territory but differed strongly from the Xi territory with resp ect to RF and surface area (P < 0.001).