INVESTIGATIONS OF RECEPTOR-MEDIATED PHAGOCYTOSIS BY HORMONE-INDUCED (IMPRINTED) TETRAHYMENA-PYRIFORMIS

Citation
P. Kovacs et al., INVESTIGATIONS OF RECEPTOR-MEDIATED PHAGOCYTOSIS BY HORMONE-INDUCED (IMPRINTED) TETRAHYMENA-PYRIFORMIS, Experientia, 52(8), 1996, pp. 769-773
Citations number
30
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Multidisciplinary Sciences
Journal title
ISSN journal
0014-4754
Volume
52
Issue
8
Year of publication
1996
Pages
769 - 773
Database
ISI
SICI code
0014-4754(1996)52:8<769:IORPBH>2.0.ZU;2-G
Abstract
Receptor-mediated endocytosis by Tetrahvmena pyriformis was studied us ing tetramethylrhodamine isothiocyanate-labeled concanavalin A (TRTTC- Con A) with fluorescence and confocal microscopy. In the presence of i nsulin, or 24 h after insulin pretreatment (hormonal imprinting), the binding and uptake of TRITC-Con A increased when compared to controls, owing to the binding of TRITC-Con A to sugar oligomers of insulin rec eptors. Mannose inhibited the binding of Con A, thus demonstrating the specificity of binding. Histamine, a phagocytosis-promoting factor in mammals and Tetrahymena, and galactose, did not influence the uptake of TRITC-Con A.