EFFECTS OF UNSATURATED FATTY-ACIDS ON EXPRESSION OF EARLY RESPONSE GENES IN HUMAN T-LYMPHOCYTES

Citation
Wv. Williams et al., EFFECTS OF UNSATURATED FATTY-ACIDS ON EXPRESSION OF EARLY RESPONSE GENES IN HUMAN T-LYMPHOCYTES, Pathobiology, 64(1), 1996, pp. 27-31
Citations number
12
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell Biology",Pathology
Journal title
ISSN journal
1015-2008
Volume
64
Issue
1
Year of publication
1996
Pages
27 - 31
Database
ISI
SICI code
1015-2008(1996)64:1<27:EOUFOE>2.0.ZU;2-G
Abstract
Administration of gamma-linolenic acid, which is converted rapidly to dihomo-gamma-linolenic acid (DGLA), reduces joint swelling and tendern ess in patients with rheumatoid arthritis. Joint tissue inflammation i n patients with rheumatoid arthritis is due in part to activation of T lymphocytes. DGLA suppresses T cell activation and production of inte rleukin-2 (IL2). The protooncogenes c-myc and c-fos are early response genes which are critical to regulation of T cell proliferation. We th erefore examined the effects of gamma-linolenic acid and other unsatur ated fatty acids on c-myc and c-fos expression by means of the polymer ase chain reaction and Northern blotting. IL2 production by the human T cell line Jurkat is dependent on a fall in mRNA for c-myc and a rise in mRNA for c-fos. The data presented here indicate that reduction of steady-state levels of mRNA for c-myc and rises in steady-state level s of mRNA for c-fos are both reduced markedly in cells incubated with DGLA. Cells incubated with arachidonic acid, eicosapentaenoic acid or oleic acid exhibit more modest changes in expression of these early re sponse genes.