IMMUNOPHENOTYPIC FEATURES AND CONFIGURATION OF IMMUNOGLOBULIN GENES IN HAIRY-CELL LEUKEMIA JAPANESE VARIANT

Citation
M. Yamaguchi et al., IMMUNOPHENOTYPIC FEATURES AND CONFIGURATION OF IMMUNOGLOBULIN GENES IN HAIRY-CELL LEUKEMIA JAPANESE VARIANT, Leukemia, 10(8), 1996, pp. 1390-1394
Citations number
30
Language
INGLESE
art.tipo
Letter
Categorie Soggetti
Hematology,Oncology
Journal title
ISSN journal
0887-6924
Volume
10
Issue
8
Year of publication
1996
Pages
1390 - 1394
Database
ISI
SICI code
0887-6924(1996)10:8<1390:IFACOI>2.0.ZU;2-K
Abstract
Immunophenotypes and Ig gene rearrangements were investigated in 12 pa tients with a variant form of hairy cell leukemia (HCL) termed HCL-Jap anese Variant (HCL-J), and in an HCL-J-derived cell line. The leukemic cells of HCL-J characteristically showed the phenotype of CD20(+), CD 5(-), CD10(-), CD11c(+), CD22(+), CD24(-) and CD25(-). Ig light (L) ch ain was undetected in nine cases, and the remaining four cases express ed kappa chain. Expression of Ig heavy (H) chain was studied in nine c ases. In addition to Ig kappa(+) cases showing expression of predomina ntly gamma H chain isotype, alpha chain was detected in one case witho ut expression of L chain. Rearranged bands in Ig heavy chain (JH) gene s were recognized in all 12 cases tested. Rearranged bands in kappa ch ain genes and germline configuration in lambda chain genes were seen i n all three Ig kappa(+) cases tested. Four of nine cases without expre ssion of L chain had a rearranged lambda chain gene. The other three c ases had lambda chain genes in the germline configuration and rearrang ed and/or deleted kappa chain genes. In the remaining two cases, no re arrangement in either kappa or lambda chain genes was detected. The Ig gene configuration and expression in HCL-J, partially overlapping wit h those described for immature B cell leukemia, were dissociated from the cytological features and CD20(+), membrane CD22(+) phenotype chara cteristic of mature B cells.