PROTECTIVE EFFECTS OF TROLOX-C, VITAMIN-C, AND CATALASE ON BROMOBENZENE-INDUCED DAMAGE TO RAT HEPATOCYTES

Citation
J. Wu et al., PROTECTIVE EFFECTS OF TROLOX-C, VITAMIN-C, AND CATALASE ON BROMOBENZENE-INDUCED DAMAGE TO RAT HEPATOCYTES, Scandinavian journal of gastroenterology, 31(8), 1996, pp. 797-803
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Gastroenterology & Hepatology
ISSN journal
0036-5521
Volume
31
Issue
8
Year of publication
1996
Pages
797 - 803
Database
ISI
SICI code
0036-5521(1996)31:8<797:PEOTVA>2.0.ZU;2-Y
Abstract
Background/Methods: The protective effects of trolox C (water-soluble vitamin E), vitamin C, and catalase on bromobenzene (BB)-induced toxic ity to isolated rat hepatocytes were evaluated. The glutathione (GSH) content of the hepatocytes exposed to BE was measured. Results: BE cau sed acute damage to the cells during 2 h of incubation (short) when BE was added directly to the culture wells, whereas a late-occurring and time-dependent increase in Lactate dehydrogenase (LDH) leakage rate w as observed during 24 h of incubation (long) when BE was dissolved in a different way. Incubation of the cells with trolox C (0.5-2.0 mM) pr evented the hepatocellular damage induced by BE at 2.4 mM during the l ong-term incubation. Vitamin C (0.1-1.0 mM) had a protective effect on BE-induced toxicity during both the short- (BE, 1.6 mM) and the long- (BB, 2.4 mM) term incubations. Catalase (3200 U/ml) also showed a ben eficial effect on the cells during the short-term BE exposure. Trolox C (2.0 mM) and vitamin C (0.5 mM) restored BE-induced GSH depletion in the cells. Conclusions: BE induced two patterns of LDH leakage from i solated hepatocytes on the basis of different ways of BE exposure and incubation periods. Trolox C, vitamin C, and catalase exerted protecti ve effects on BE-induced toxicity during short- or/and long-term incub ations. The effects were concentration-dependent. Restoration of GSH c ontent in BE-exposed hepatocytes suggests that trolox C and vitamin C could reduce GSH consumption during BE metabolism and exert an antioxi dant effect.