THE ROLE OF FREE-RADICALS IN P-AMINOPHENOL-INDUCED NEPHROTOXICITY - DOES REDUCED GLUTATHIONE HAVE A PROTECTIVE EFFECT

Citation
G. Kanbak et al., THE ROLE OF FREE-RADICALS IN P-AMINOPHENOL-INDUCED NEPHROTOXICITY - DOES REDUCED GLUTATHIONE HAVE A PROTECTIVE EFFECT, Clinica chimica acta, 252(1), 1996, pp. 61-71
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry Medicinal",Biology
Journal title
ISSN journal
0009-8981
Volume
252
Issue
1
Year of publication
1996
Pages
61 - 71
Database
ISI
SICI code
0009-8981(1996)252:1<61:TROFIP>2.0.ZU;2-H
Abstract
The role of free radicals in p-aminophenol (PAP)-induced nephrotoxicit y and effects of reduced glutathione (GSH) were investigated. We injec ted PAP in one group of rats and PAP plus GSH in a second group. All p arameters were measured in the renal tissue. Superoxide dismutase (SOD ) activity in the PAP + GSH group (7.1 +/- 0.36 U/mg protein) was foun d to be significantly higher than in the control group (4.9 +/- 0.13) (P < 0.001). Catalase (CAT) was found to be significantly low in both groups (P < 0.001 in the PAP group (13.48 +/- 0.85 U/mg protein), P < 0.01 in the PAP + GSH group (18.75 +/- 1.17) as compared to the contro l group (41.03 +/- 0.93)). Glutathione peroxidase (GPx) in the PAP and PAP + GSH groups was found to be significantly high (P < 0.01 in the PAP group (5.32 +/- 0.033 U/mg protein), P < 0.001 in the PAP + GSH gr oup (6.48 +/- 0.1)) as compared to the control group (2.93 +/- 0.093)) . Similarly, glutathione reductase (GSSGR) in the PAP (0.023 +/- 0.002 U/mg protein), and PAP + GSH (0.025 +/- 0.001) groups was found to be significantly high as compared to the control group (0.014 +/- 0.001) (P < 0.001). GSH in the PAP (161.93 +/- 8.3 mg/mg protein) and PAPS-G SH (170.7 +/- 4.51) groups were found to be significantly higher than the control group (104.91 +/- 3.0) (P < 0.001). Malondialdehyte (MDA) in the PAP (11.12 +/- 0.62 nmol/mg protein) and PAP +/- GSH (9.72 +/- 0.46) groups was found to be significantly higher than in the control group (5.54 +/- 0.51) (P < 0.001). Free radicals might have a major ro le in the PAP-induced nephrotoxicity, GSH increased nephrotoxicity.