INTRACELLULAR-DISTRIBUTION OF HMG1, HMG2 AND UBF CHANGE FOLLOWING TREATMENT WITH CISPLATIN

Citation
Jc. Chao et al., INTRACELLULAR-DISTRIBUTION OF HMG1, HMG2 AND UBF CHANGE FOLLOWING TREATMENT WITH CISPLATIN, Biochimica et biophysica acta, N. Gene structure and expression, 1307(2), 1996, pp. 213-219
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Biophysics,"Biothechnology & Applied Migrobiology
ISSN journal
0167-4781
Volume
1307
Issue
2
Year of publication
1996
Pages
213 - 219
Database
ISI
SICI code
0167-4781(1996)1307:2<213:IOHHAU>2.0.ZU;2-P
Abstract
Cisplatin (CDDP) is a widely used cancer chemotherapeutic agent. CDDP forms well characterized intrastrand cross-links between adjacent puri nes in genomic DNA. In mammalian cells, these lesions are repaired by the nucleotide excision repair system. An early event in the recogniti on and processing of cis-Pt-DNA adducts may well involve the binding o f specific proteins to the sites of damage. Several proteins have been identified, including high mobility group (HMG) proteins 1 and 2 and upstream binding factor (UBF), which recognize CDDP-DNA. However, the physiological significance of this binding has not been established. I n this study, we have utilized antibodies to these proteins to examine the effect of CDDP on their intracellular distribution. Marked change s in the immunofluorescent staining pattern of HMG1/HMG2 were noted in cells treated with CDDP. At higher drug concentrations, the distribut ion of UBF also changed, from a clustered appearance associated with t he nucleoli to more diffuse nuclear staining, These results demonstrat e that HMG1/HMG2 and UBF respond to drug treatment, presumably by reco gnizing cis-Pt-DNA adduct formation in intact cells. Hence, these prot eins may play an important role in directing the response of tumor cel ls following exposure to CDDP.