CRYSTALLIZATION OF A NOVEL ESTERASE WHICH INACTIVATES THE MACROLIDE TOXIN BREFELDIN-A

Citation
Y. Wei et al., CRYSTALLIZATION OF A NOVEL ESTERASE WHICH INACTIVATES THE MACROLIDE TOXIN BREFELDIN-A, Acta crystallographica. Section D, Biological crystallography, 52, 1996, pp. 1194-1195
Citations number
12
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biology
ISSN journal
0907-4449
Volume
52
Year of publication
1996
Part
6
Pages
1194 - 1195
Database
ISI
SICI code
0907-4449(1996)52:<1194:COANEW>2.0.ZU;2-5
Abstract
A novel esterase obtained from Bacillus subtilis and capable of hydrol yzing the phytotoxin brefeldin A was crystallized using the hanging-dr op technique. The crystals have two forms and both are monoclinic: for m I, space group P2(1) with a = 101.7. b = 64.1, c = 55.4 Angstrom and B = 102.5 degrees, and form II, space group C2. with a = 140.7, b = 8 2.6, c = 81.5 Angstrom and beta = 112.5 degrees. There are two molecul es related by a pronounced non-crystallographic dyad per asymmetric un it in both crystal forms. The crystals diffract to 2.3 Angstrom using a rotating-anode X-ray source.