CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC ANALYSIS OF CABBAGE HISTIDINOL DEHYDROGENASE

Citation
Sw. Cowanjacob et al., CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC ANALYSIS OF CABBAGE HISTIDINOL DEHYDROGENASE, Acta crystallographica. Section D, Biological crystallography, 52, 1996, pp. 1188-1190
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biology
ISSN journal
0907-4449
Volume
52
Year of publication
1996
Part
6
Pages
1188 - 1190
Database
ISI
SICI code
0907-4449(1996)52:<1188:CAPCAO>2.0.ZU;2-I
Abstract
Recombinant Brassica oleracea histidinol dehydrogenase (HDH) has been crystallized in various space groups using the method of vapour diffus ion. The presence or absence of inhibitors and substrates as well as t he use of different precipitants has enabled the growth of five differ ent crystal forms. Extensive searches with the first crystal form (A) failed to produce any useful heavy-atom derivatives, mainly because of the instability of the crystals. This provoked the search for further crystal forms in the hope of finding more suitable crystals. At least two of these crystal forms are of interest for further study.