PRELIMINARY INVESTIGATION OF GLYCOSYLATED PROTEINS BY CAPILLARY ELECTROPHORESIS AND CAPILLARY ELECTROPHORESIS MASS-SPECTROMETRY USING ELECTROSPRAY-IONIZATION AND BY MATRIX-ASSISTED LASER-DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY

Citation
R. Bonfichi et al., PRELIMINARY INVESTIGATION OF GLYCOSYLATED PROTEINS BY CAPILLARY ELECTROPHORESIS AND CAPILLARY ELECTROPHORESIS MASS-SPECTROMETRY USING ELECTROSPRAY-IONIZATION AND BY MATRIX-ASSISTED LASER-DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY, Journal of mass spectrometry., 1995, pp. 95-106
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry Inorganic & Nuclear",Spectroscopy,Biophysics
ISSN journal
1076-5174
Year of publication
1995
Supplement
S
Pages
95 - 106
Database
ISI
SICI code
1076-5174(1995):<95:PIOGPB>2.0.ZU;2-#
Abstract
In recent years, the biotechnological industry has emerged as the majo r source of new human therapeutic proteins. Although the great majorit y of these occur naturally as glycoproteins, it has been observed that glycosylation of the recombinantly produced proteins could be fundame ntal for their in vivo activity (e.g. tissue plasminogen activator, er ythropoietin) or, on the contrary, insignificant (e.g. interleukin-1 r eceptor antagonist, gamma-interferon, granulocyte macrophage-colony-st imulating factor). The inherent heterogeneity of these complex biomole cules presents an exciting challenge in the analytical field for both their structural analysis and the development of suitable analytical m ethods to guarantee consistency of their production. Owing to this eve r increasing therapeutic interest in proteins and glycoproteins, this paper compares the information provided by different analytical techni ques (i.e. high-performance liquid chromatography, sodium dodecyl sulp hate polyacrylamide gel electrophoresis, capillary electrophoresis, ca pillary electrophoresis/electrospray ionization mass spectrometry, mat rix-assisted laser desorption/ionization time-of flight mass spectrome try and high-performance liquid chromatography/matrix-assisted laser d esorption/ionization time-of flight mass spectrometry when used for th e analysis of proteins and glycoproteins. For the sake of simplicity, reference standard proteins and glycoproteins were used as samples.