PRELIMINARY INVESTIGATION OF GLYCOSYLATED PROTEINS BY CAPILLARY ELECTROPHORESIS AND CAPILLARY ELECTROPHORESIS MASS-SPECTROMETRY USING ELECTROSPRAY-IONIZATION AND BY MATRIX-ASSISTED LASER-DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY
R. Bonfichi et al., PRELIMINARY INVESTIGATION OF GLYCOSYLATED PROTEINS BY CAPILLARY ELECTROPHORESIS AND CAPILLARY ELECTROPHORESIS MASS-SPECTROMETRY USING ELECTROSPRAY-IONIZATION AND BY MATRIX-ASSISTED LASER-DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY, Journal of mass spectrometry., 1995, pp. 95-106
In recent years, the biotechnological industry has emerged as the majo
r source of new human therapeutic proteins. Although the great majorit
y of these occur naturally as glycoproteins, it has been observed that
glycosylation of the recombinantly produced proteins could be fundame
ntal for their in vivo activity (e.g. tissue plasminogen activator, er
ythropoietin) or, on the contrary, insignificant (e.g. interleukin-1 r
eceptor antagonist, gamma-interferon, granulocyte macrophage-colony-st
imulating factor). The inherent heterogeneity of these complex biomole
cules presents an exciting challenge in the analytical field for both
their structural analysis and the development of suitable analytical m
ethods to guarantee consistency of their production. Owing to this eve
r increasing therapeutic interest in proteins and glycoproteins, this
paper compares the information provided by different analytical techni
ques (i.e. high-performance liquid chromatography, sodium dodecyl sulp
hate polyacrylamide gel electrophoresis, capillary electrophoresis, ca
pillary electrophoresis/electrospray ionization mass spectrometry, mat
rix-assisted laser desorption/ionization time-of flight mass spectrome
try and high-performance liquid chromatography/matrix-assisted laser d
esorption/ionization time-of flight mass spectrometry when used for th
e analysis of proteins and glycoproteins. For the sake of simplicity,
reference standard proteins and glycoproteins were used as samples.