ENTERIC PATHOGENS ASSOCIATED WITH GASTROINTESTINAL DYSFUNCTION IN CHILDREN WITH HIV-INFECTION

Citation
Ag. Ramossoriano et al., ENTERIC PATHOGENS ASSOCIATED WITH GASTROINTESTINAL DYSFUNCTION IN CHILDREN WITH HIV-INFECTION, Molecular and cellular probes, 10(2), 1996, pp. 67-73
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell Biology",Biology,"Biochemical Research Methods
ISSN journal
0890-8508
Volume
10
Issue
2
Year of publication
1996
Pages
67 - 73
Database
ISI
SICI code
0890-8508(1996)10:2<67:EPAWGD>2.0.ZU;2-O
Abstract
Infants and young children with HIV infection commonly suffer from gas trointestinal manifestations of their disease. Many HIV infected child ren have evidence of persistent diarrhoea, malabsorption, malnutrition or growth failure. The aetiology and pathogenesis of gastrointestinal dysfunction in HIV infected children have not been well defined. We p erformed immunocytochemical analyses on intestinal tissue from 19 HIV- infected children with gastrointestinal dysfunction or growth failure. None of these 19 children had microbial pathogens identified in faeca l samples using standard microbiological methods. Intestinal tissues w ere obtained from the children by biopsy and were examined for antigen s from Pneumocystis carinii, cytomegalovirus (CMV) and herpes simplex virus (HSV) using the avidin-biotin-complex immunohistochemical techni que and monoclonal or monospecific antibodies. We detected at least on e of these pathogens in samples from eight (42%) of 19 HIV infected ch ildren. P. carinii was the most prevalent pathogen, found in five of t he eight HIV infected children. All of the children with intestinal pn eumocystis infection were receiving prophylaxis directed at the preven tion of pulmonary disease with this organism and none of them were und ergoing active pulmonary infection. We also identified CMV antigens in intestinal tissues from four children and HSV antigens in intestinal tissues from one child. Two children were infected with more than one pathogen. On the other hand, none of these pathogens were found in the tissues obtained from 10 HIV-uninfected patients who had intestinal t issues obtained for chronic non-infectious diarrheal and inflammatory diseases (P<0.01, Fisher's exact test). Our findings indicate that som e children with HIV infection and gastrointestinal dysfunction may be infected with opportunistic pathogens despite negative analyses employ ing standard microbiological methods. Our study also indicates that HI V infected children can undergo intestinal infection with Fl carinii d espite the administration of standard immunoprophylactic regimens dire cted at the prevention of infection with this organism. (C) 1996 Acade mic Press Limited