ULTRASTRUCTURE OF HEPATOCYTES IN COPPER-DEFICIENT SIKA-DEER (CERVUS-NIPPON TEMMINCK)

Citation
H. Seo et al., ULTRASTRUCTURE OF HEPATOCYTES IN COPPER-DEFICIENT SIKA-DEER (CERVUS-NIPPON TEMMINCK), Journal of Comparative Pathology, 114(3), 1996, pp. 283-290
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pathology,"Veterinary Sciences
ISSN journal
0021-9975
Volume
114
Issue
3
Year of publication
1996
Pages
283 - 290
Database
ISI
SICI code
0021-9975(1996)114:3<283:UOHICS>2.0.ZU;2-I
Abstract
The livers of 13 Sika deer (Cervus nippon Temminck) aged 4 to 9 years and suffering from copper deficiency (enzootic ataxia) were examined h istologically, histochemically and by electron microscopy. In addition , the serum and liver copper concentrations, measured in three animals , were found to be low. Histologically, the hepatocytes exhibited clou dy swelling, and numerous haemosiderin deposits were seen in the hepat ocytes and Kupffer cells. Staining with p-dimethyl amino-benzylidene-r hodamine revealed distinctly fewer copper granules than normal. Histoc hemically, 3,3'-diaminobenzidine-H2O2 staining revealed increased numb ers of catalase-positive granules around nuclei. Electron microscopica lly, ''giant'' and bizarre-shaped mitochondria; irregular depression o f the mitochondrial membrane, and fusion of cristae were noted. Disord ers of copper-containing enzymes, including cytochrome oxidase, caerul oplasmin and monoamine oxidase, may have been responsible for the mito chondrial abnormalities. (C) 1996 W.B. Saunders Company Limited