ROLE OF MASS-SPECTROMETRY IN VITAMIN-D RESEARCH

Authors
Citation
B. Yeung et P. Vouros, ROLE OF MASS-SPECTROMETRY IN VITAMIN-D RESEARCH, Mass spectrometry reviews, 14(3), 1995, pp. 179-194
Citations number
72
Language
INGLESE
art.tipo
Review
Categorie Soggetti
Spectroscopy
Journal title
ISSN journal
0277-7037
Volume
14
Issue
3
Year of publication
1995
Pages
179 - 194
Database
ISI
SICI code
0277-7037(1995)14:3<179:ROMIVR>2.0.ZU;2-L
Abstract
1,25-dihydroxyvitamin D-3 is the active metabolite of vitamin D-3, and has been found to play important roles in mineral balance and immunol ogical regulation. Recently, 1,25-dihydroxyvitamin D-3 and similar syn thetic analogs in development have been found to possess potential the rapeutic values in the treatment of proliferative diseases, including leukemia, breast cancer, and psoriasis. Current methods for the analys is of these compounds may either lack the necessary sensitivity (i.e., UV detection) or specificity (i.e., receptor binding assay). In addit ion, the synthetic analogs developed to date have nor been found to un iformly bind with receptors in binding assays, a property that may ren der them to be difficult for measurement at the trace levels. Methods utilizing mass spectrometry (MS) have been used for either structural identification or quantification. Electron impact ionization has been the technique of choice for structural analysis, whereas newer ionizat ion techniques have been utilized for the quantification of metabolite s, often in conjunction with on-line separation. In this review, MS an d non-MS based methods for vitamin D analysis will be discussed, with an emphasis on the former. Historical perspectives, the present state, and the future role of MS in vitamin D research will be discussed. (C ) 1996 John Wiley & Sons, Inc.