EFFECTS OF PERTUSSIS TOXIN ON SIGNAL TRANSDUCTIONS VIA P-2-PURINERGICRECEPTORS IN A-431 HUMAN EPIDERMOIDAL CARCINOMA-CELLS

Citation
K. Sugita et al., EFFECTS OF PERTUSSIS TOXIN ON SIGNAL TRANSDUCTIONS VIA P-2-PURINERGICRECEPTORS IN A-431 HUMAN EPIDERMOIDAL CARCINOMA-CELLS, Enzyme & protein, 48(4), 1995, pp. 222-228
Citations number
18
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology
Journal title
ISSN journal
1019-6773
Volume
48
Issue
4
Year of publication
1995
Pages
222 - 228
Database
ISI
SICI code
1019-6773(1995)48:4<222:EOPTOS>2.0.ZU;2-2
Abstract
In A-431 cells, stimulation of P-2-purinergic receptors with extracell ular ATP caused production of inositol 1,4,5-trisphosphate (InsP(3)), followed by mobilization of Ca2+ from intracellular stores; Ca2+ influ x from the extracellular fluid and breakdown of phosphatidylcholine (P tdCho) also accompanied this InsP(3)/Ca2+ signalling. When A-431 cells were pretreated with pertussis toxin (PTX), production of InsP(3) and elevation of cytosolic Ca2+ were strongly inhibited. PTX also inhibit ed the Ca2+ influx, but the effect was much weaker than that for InsP( 3)/Ca2+ elevation. No inhibitory effect was observed in ATP-stimulated PtdCho breakdown. These results suggest that there is a system(s) whi ch mediates the functions of P-2-purinergic receptors in addition to P TX-sensitive G-proteins.