EFFECTS OF NERVE GROWTH-FACTOR ON X-IRRADIATED REAGGREGATION CULTURESOF RAT-BRAIN CELLS

Citation
Y. Dimberg et al., EFFECTS OF NERVE GROWTH-FACTOR ON X-IRRADIATED REAGGREGATION CULTURESOF RAT-BRAIN CELLS, International journal of radiation biology, 64(6), 1993, pp. 731-737
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Radiology,Nuclear Medicine & Medical Imaging","Nuclear Sciences & Tecnology
ISSN journal
0955-3002
Volume
64
Issue
6
Year of publication
1993
Pages
731 - 737
Database
ISI
SICI code
0955-3002(1993)64:6<731:EONGOX>2.0.ZU;2-6
Abstract
The effects of exogenously added nerve growth factor (NGF) on reaggreg ation cultures of foetal rat brain cells after X-irradiation with 2 Gy were studied. Irradiation caused decreased protein and DNA levels, wh ich was not prevented by NGF. The activities of the cholinergic marker enzymes choline acetyl transferase and acetylcholine esterase were in creased in irradiated cultures. However, no difference in the activiti es of these enzymes was found between irradiated and unirradiated NGF- treated cultures. Irradiation did not affect the activity of the marke r enzyme for oligodendrocytes (2',3'-cyclic nucleotide 3'-phosphodiest erase), but caused an increase in the astrocyte marker (glutamine synt hetase) activity. This effect on astrocytes was prevented by NGF.