TRANSFORMATION-SENSITIVE CELLS IN G2 M-PHASE ARE NOT PROMOTED BY TPA FOLLOWING CS-137 GAMMA-RAYS/

Citation
Rl. Wells et al., TRANSFORMATION-SENSITIVE CELLS IN G2 M-PHASE ARE NOT PROMOTED BY TPA FOLLOWING CS-137 GAMMA-RAYS/, International journal of radiation biology, 64(6), 1993, pp. 727-730
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Radiology,Nuclear Medicine & Medical Imaging","Nuclear Sciences & Tecnology
ISSN journal
0955-3002
Volume
64
Issue
6
Year of publication
1993
Pages
727 - 730
Database
ISI
SICI code
0955-3002(1993)64:6<727:TCIGMA>2.0.ZU;2-Q
Abstract
Mouse C3H 10T1/2 cells are most sensitive to radiation-induced neoplas tic transformation in the G2/M-phase of the cell cycle. When synchroni zed 10T1/2 cells were exposed to phorbol 12-myristate 13-acetate (TPA) after irradiation, transformation of cells not in the transformation- sensitive window was enhanced, but transformation of cells already in the transformation-sensitive window was not. Earlier work showed that (a) TPA enhances the frequency of transformation of both high and low dose-rate gamma-irradiated cells by about the same factor, but that (b ) TPA enhances the transformation of cells exposed to low dose-rate fi ssion spectrum neutrons appreciably less than cells exposed to high do se-rate fission spectrum neutrons. The latter observation is consisten t with the inability of TPA to promote cells in the transformation-sen sitive window and with the role of such cells in enhancing transformat ion by protracted doses of neutrons. The data provide a cellular basis for studying the biochemical/molecular aspects of TPA promotion in vi tro.