ACETALDEHYDE INVOLVEMENT IN ETHANOL-INDUCED POTENTIATION OF RAT HEPATOCYTE DAMAGE DUE TO THE CARCINOGEN 1,2-DIBROMOETHANE

Citation
E. Chiarpotto et al., ACETALDEHYDE INVOLVEMENT IN ETHANOL-INDUCED POTENTIATION OF RAT HEPATOCYTE DAMAGE DUE TO THE CARCINOGEN 1,2-DIBROMOETHANE, Alcohol and alcoholism, 30(6), 1995, pp. 721-728
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Substance Abuse
Journal title
ISSN journal
0735-0414
Volume
30
Issue
6
Year of publication
1995
Pages
721 - 728
Database
ISI
SICI code
0735-0414(1995)30:6<721:AIIEPO>2.0.ZU;2-9
Abstract
Previous experiments with hepatocytes isolated from ethanol-treated ra ts showed that alcohol potentiates the toxic action of 1,2-dibromoetha ne (DBE) by inhibiting its metabolism via glutathione-S-transferase. T he aim of this study was to investigate whether acetaldehyde, the main product of ethanol metabolism, may be responsible for such inactivati on. By pretreatment with 4-methylpyrazole, an inhibitor of acetaldehyd e formation, the ethanol inactivation of glutathione transferase was a ctually prevented. As a consequence of this protective action, 4-methy lpyrazole also prevented the high basal lipid peroxidation and the pot entiated DBE toxicity observed in hepatocytes from ethanol-dosed anima ls. Finally, the inactivation of glutathione-S-transferase by concentr ations of acetaldehyde likely to occur in the ethanol-intoxicated anim al was confirmed in an in vitro model by direct aldehyde addition to h epatocyte suspensions.