THE INFLUENCE OF CULTURED SCHWANN-CELLS ON REGENERATION THROUGH ACELLULAR BASAL LAMINA GRAFTS

Citation
Ak. Gulati et al., THE INFLUENCE OF CULTURED SCHWANN-CELLS ON REGENERATION THROUGH ACELLULAR BASAL LAMINA GRAFTS, Brain research, 705(1-2), 1995, pp. 118-124
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
0006-8993
Volume
705
Issue
1-2
Year of publication
1995
Pages
118 - 124
Database
ISI
SICI code
0006-8993(1995)705:1-2<118:TIOCSO>2.0.ZU;2-U
Abstract
Acellular basal lamina grafts have been shown to be less immunogenic i n comparison to cellular grafts, but possess a limited potential for s upporting axonal regeneration through them. The present study describe s the effect of cultured Schwann cells on enhancing regeneration throu gh acellular grafts. 2 cm long acellular grafts, and in vitro Schwann cell populated acellular grafts were used to repair a surgically creat ed gap in the host peroneal nerve. The transplants were analyzed at 1, 2, 4 and 8 weeks to determine their ability to support axonal regener ation. Host axonal regeneration through Schwann cell cocultured acellu lar grafts occurred rapidly and was significantly better as compared t o non-cultured acellular grafts. The results demonstrate a beneficial effect of Schwann cell culture pretreatment on regeneration through ac ellular grafts and an improved recovery of the target muscle. The proc edure of first preparing acellular grafts with subsequent coculture wi th Schwann cells offers a novel approach for the repair of injured ner vous tissue.