PERMANENT ALTERATION OF MUSCARINIC ACETYLCHOLINE-RECEPTOR BINDING IN RAT STRIATUM AFTER CIRCLING TRAINING DURING DEVELOPMENT

Citation
Gr. Ibarra et al., PERMANENT ALTERATION OF MUSCARINIC ACETYLCHOLINE-RECEPTOR BINDING IN RAT STRIATUM AFTER CIRCLING TRAINING DURING DEVELOPMENT, Brain research, 705(1-2), 1995, pp. 39-44
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
0006-8993
Volume
705
Issue
1-2
Year of publication
1995
Pages
39 - 44
Database
ISI
SICI code
0006-8993(1995)705:1-2<39:PAOMAB>2.0.ZU;2-B
Abstract
We evaluated the effect of circling training (CT) in the expression of muscarinic acetylcholine receptor (mAchR) in developing rat striatum. For this, male and female rats were subjected to CT at 20, 30, 40 and 60 days of age during 7 days. Animals trained at 30 days but not at o ther ages showed an average decreased binding to mAchR of 33% in males and 24% in females, representing a significant difference with respec t to control non-trained animals (males P < 0.001, females P < 0.005), and showing also a differential response between sex (P < 0.01). mAch R drop was found invariably either 2 months or 1 year after training i ndicating a long term plastic change due to circling training. Scatcha rd analysis showed that altered binding represents a variation of the total receptor number instead of its binding affinity, with no signifi cant differences found among K-d (P > 0.1). mAchR variation was correl ated with the motor performance accomplished in the test. Regarding to tal distance run, male rats trained for 3 days (300 meters run), for 5 days (600 meters) and for 7 days (900 meters) showed a drop of 19, 28 and 33% respectively (r(2) = 0.91, P < 0.001), while female changes w ere of 21, 23 and 24% (r(2) = 0.78, P < 0.001). Nevertheless, no corre lation with running speed was found (r(2) = 0.13 male, r(2) = 0.02 fem ale; P > 0.1). In summary, these results demonstrate the presence of a limited sensitivity period during striatum development where mAchR ex pression may be affected by the activity performed during CT, represen ting a permanent alteration of the receptor levels.