RETROGRADE TRANSFER OF REPLICATION DEFICIENT RECOMBINANT ADENOVIRUS VECTOR IN THE CENTRAL-NERVOUS-SYSTEM FOR TRACING STUDIES

Citation
H. Kuo et al., RETROGRADE TRANSFER OF REPLICATION DEFICIENT RECOMBINANT ADENOVIRUS VECTOR IN THE CENTRAL-NERVOUS-SYSTEM FOR TRACING STUDIES, Brain research, 705(1-2), 1995, pp. 31-38
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
0006-8993
Volume
705
Issue
1-2
Year of publication
1995
Pages
31 - 38
Database
ISI
SICI code
0006-8993(1995)705:1-2<31:RTORDR>2.0.ZU;2-5
Abstract
We assessed the application of a replication deficient recombinant ade novirus vector as a retrograde tracer in neural pathway studies. The a denovirus vector, Ad.RSV beta gal, containing the intracellular marker gene, B-galactosidase, was injected directly into the laterodorsal st riatum of rats. The retrograde transport of the vector from the inject ion site was clearly visible in the cerebral cortex, thalamic nucleus, and substantia nigra. No evidence for anterograde transport of the ve ctor was found. When the vector was injected into the genu of the corp us callosum, little uptake of the vector by fibers was noted which sug gested that uptake by fibers-of-passage should not be a problem in tra cing studies. The present study demonstrates that adenoviral vectors c an be useful retrograde tracers in the study of afferent connections w ithin the central nervous system.