Bl. Lim et U. Holmskov, EXPRESSION OF THE CARBOHYDRATE-RECOGNITION DOMAIN OF BOVINE CONGLUTININ AND DEMONSTRATION OF ITS BINDING TO IC3B AND YEAST MANNAN, Biochemical and biophysical research communications, 218(1), 1996, pp. 260-266
Bovine conglutinin is a collagenous C-type lectin (collectin) that is
found in bovine serum. A recombinant protein, composed of the neck-reg
ion and the carbohydrate binding domain of bovine conglutinin, has bee
n overexpressed in E. coli. The recombinant protein has been successfu
lly renatured and showed the same sugar binding specificity as the nat
ive protein and was able to bind to yeast mannan and complement-activa
ted immune complexes. The binding was calcium-dependent and was inhibi
ted by N-acetylglucosamine. The concentration of N-acetylglucosamine r
equired for 50% inhibition of binding to mannan was 1.77 mM for recomb
inant conglutinin and 0.71 mM for native conglutinin, respectively. Th
e recombinant conglutinin should be useful in the assay and purificati
on of circulating immune complexes and for therapeutic purposes involv
ing the removal of immune complexes from patient's plasma. (C) 1996 Ac
ademic Press, Inc.