NITRATE REDUCTION IN A NEW STRAIN OF RHODOFERAX FERMENTANS

Citation
A. Hougardy et Jh. Klemme, NITRATE REDUCTION IN A NEW STRAIN OF RHODOFERAX FERMENTANS, Archives of microbiology, 164(5), 1995, pp. 358-362
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
ISSN journal
0302-8933
Volume
164
Issue
5
Year of publication
1995
Pages
358 - 362
Database
ISI
SICI code
0302-8933(1995)164:5<358:NRIANS>2.0.ZU;2-S
Abstract
Using an enrichment technique avoiding the dominating fast-growing spe cies Rhodobacter capsulatus and Rhodobacter sphaeroides, a new strain (designated B2c; DSM 10 139) of Rhodoferax fermentans, a facultatively phototrophic member of the beta subclass of Proteobacteria, was isola ted. In contrast to the type strain of this species, strain B2c was ca pable of reducing nitrate. Strain B2c contained a highly active nitrat e reductase [0.3-0.4 mu mol min(-1) (mg protein)(-1)] which, in extrac ts from spheroplasts prepared by ultrasonic treatment, was associated with the membrane fraction. The physiological role of nitrate reductas e depended on the growth conditions. The enzyme enabled the strain to grow with nitrate as a nitrogen source and to maintain redox balance i n a culture medium with a highly reduced carbon compound.