SELF-REACTIVE CYTOTOXIC GAMMA-DELTA T-LYMPHOCYTES IN GRAVES-DISEASE SPECIFICALLY RECOGNIZE THYROID EPITHELIAL-CELLS

Citation
M. Catalfamo et al., SELF-REACTIVE CYTOTOXIC GAMMA-DELTA T-LYMPHOCYTES IN GRAVES-DISEASE SPECIFICALLY RECOGNIZE THYROID EPITHELIAL-CELLS, The Journal of immunology, 156(2), 1996, pp. 804-811
Citations number
49
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
The Journal of immunology
ISSN journal
0022-1767 → ACNP
Volume
156
Issue
2
Year of publication
1996
Pages
804 - 811
Database
ISI
SICI code
0022-1767(1996)156:2<804:SCGTIG>2.0.ZU;2-T
Abstract
In this paper we report the isolation of a self-reactive cytotoxic gam ma delta T cell line, 158RE.2, that originates from the T lymphocyte p opulation infiltrating the thyroid gland of a patient with Graves' dis ease. Functional data using this cell line demonstrate that gamma delt a T cells expanded in the thyroid tissue specifically recognize a liga nd expressed by thyroid epithelial cells and cell lines of endocrine e pithelial origin, The TCR expressed by these gamma delta T cells-V gam ma 1/V delta 5-is unusual in peripheral blood lymphocytes, and its spe cificity is clearly different from that observed in a high percentage of gamma delta T cells from PBL, which express the common TCR V gamma 9/V delta 2, The V gamma 1/V gamma 5 receptor is involved in the recog nition of the ligand expressed by the thyroid cells, but not in the NK -like activity also displayed by 158RE.2, These cells express CD8 alph a alpha dimers, which participate in the thyroid ligand recognition bu t not in the NK-like activity, The epithelial cell recognition is not restricted by classical MHC class I or class II molecules, although th e CD8 alpha alpha participation in the recognition suggests the involv ement of nonclassical MHC molecules, These are the first data to be pr esented on self-reacting gamma delta T cells in human epithelium.