POLYSACCHARIDE-MEDIATED PROTECTION AGAINST ABSCESS FORMATION IN EXPERIMENTAL INTRAABDOMINAL SEPSIS

Citation
Ao. Tzianabos et al., POLYSACCHARIDE-MEDIATED PROTECTION AGAINST ABSCESS FORMATION IN EXPERIMENTAL INTRAABDOMINAL SEPSIS, The Journal of clinical investigation, 96(6), 1995, pp. 2727-2731
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medicine, Research & Experimental
ISSN journal
0021-9738
Volume
96
Issue
6
Year of publication
1995
Pages
2727 - 2731
Database
ISI
SICI code
0021-9738(1995)96:6<2727:PPAAFI>2.0.ZU;2-8
Abstract
Abscess formation is a major complication of intra-abdominal sepsis th at causes significant morbidity and mortality. In such cases, Bacteroi des fragilis is the predominant anaerobic isolate. In a rat model of i ntra-abdominal sepsis, the capsular polysaccharide complex (CPC) from B. fragilis promotes abscess formation and when administered sub-cutan eously, protects against this host response by a T cell-dependent immu ne mechanism. In the present study, the polysaccharide A (PS A) compon ent of CPC protected animals against challenge with live heterologous bacterial species (mixtures of anaerobes and facultative organisms) th at are most commonly isolated from intra-abdominal abscesses in humans . Protection against heterologous bacterial challenge was transferred by T cells. Administration of PS A shortly before or even after challe nge with B. fragilis protected against this host response. In experime nts designed to simulate fecal contamination of the human peritoneal c avity, PS A protected animals against abscess formation induced by a r at cecal contents inoculum. The surprisingly broad protective activity of PS A indicates that this molecule is likely suppressing a nonspeci fic host tissue reaction that forms in response to a variety of absces s-inducing organisms and that it might be useful in preventing abscess formation associated with intra-abdominal sepsis in the clinical sett ing.