EFFECTS OF THE APOLIPOPROTEIN-E POLYMORPHISM ON UPTAKE AND TRANSFER OF CELL-DERIVED CHOLESTEROL IN PLASMA

Citation
Yd. Huang et al., EFFECTS OF THE APOLIPOPROTEIN-E POLYMORPHISM ON UPTAKE AND TRANSFER OF CELL-DERIVED CHOLESTEROL IN PLASMA, The Journal of clinical investigation, 96(6), 1995, pp. 2693-2701
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medicine, Research & Experimental
ISSN journal
0021-9738
Volume
96
Issue
6
Year of publication
1995
Pages
2693 - 2701
Database
ISI
SICI code
0021-9738(1995)96:6<2693:EOTAPO>2.0.ZU;2-X
Abstract
The reverse cholesterol transport is initiated by the uptake of choles terol into minor subfractions of high density lipoproteins (HDL) which contain either apolipoprotein (ape) A-I or apoE as their only apolipo proteins, From these initial accepters, which are termed pre beta(1)-L pA-I and gamma-LpE, respectively, cell-derived cholesterol is transfer red to LDL via the bulk of HDL termed alpha-LpA-I, In this study we an alyzed the effect in plasma of the genetically determined apoE polymor phism on the formation of gamma-LpE, uptake and transfer of cell-deriv ed cholesterol to LDL, gamma-LpE was immunologically detectable in pla smas of individuals carrying at least one apoE3-allele but not in apoE 3-free plasmas. During one minute incubation with [H-3] cholesterol-la beled fibroblasts, gamma-LpE of plasmas from apoE3/3 subjects accumula ted 7-and 13-fold more radioactivity than the respective fractions in plasmas from apoE2/2- and apoE4/4-subjects, respectively, Totally, 30% less [3H]cholesterol was released into plasmas of apoE2/2 and apoE4/4 -individuals as compared with plasmas of apoE3/3-subjects. Moreover, p lasmas of apoE3/3 individuals accumulated 50% and 65% more cell-derive d [H-3] cholesterol in alpha-LpA-I-2 than plasmas of apoE4/4 and apoE2 /2-subjects, respectively, These results indicate that the apoE-polymo rphism is an important determinant of the uptake and transfer of cell- derived cholesterol in plasma.