ENDOGENOUS PRODUCTION OF INTERLEUKIN-15 BY ACTIVATED HUMAN MONOCYTES IS CRITICAL FOR OPTIMAL PRODUCTION OF INTERFERON-GAMMA BY NATURAL-KILLER-CELLS IN-VITRO

Citation
We. Carson et al., ENDOGENOUS PRODUCTION OF INTERLEUKIN-15 BY ACTIVATED HUMAN MONOCYTES IS CRITICAL FOR OPTIMAL PRODUCTION OF INTERFERON-GAMMA BY NATURAL-KILLER-CELLS IN-VITRO, The Journal of clinical investigation, 96(6), 1995, pp. 2578-2582
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medicine, Research & Experimental
ISSN journal
0021-9738
Volume
96
Issue
6
Year of publication
1995
Pages
2578 - 2582
Database
ISI
SICI code
0021-9738(1995)96:6<2578:EPOIBA>2.0.ZU;2-W
Abstract
Natural killer (NK) cells are large granular lymphocytes that constitu tively express functional IL-2 receptors, We have shown that recombina nt human IL-15 uses the IL-2 receptor to activate human NK cells and c an synergize with recombinant human IL-12 to stimulate NK cell product ion of IFN-gamma in vitro, IFN-gamma production by NK cells is critica l in the prevention of overwhelming infection by obligate intracellula r microbial pathogens in several experimental animal models, Herein, w e demonstrate that human monocytes produce IL-15 protein within 5 h of activation with LPS, Using an IL-15-neutralizing antiserum in a cocul ture of LPS-activated monocytes and NK cells, we demonstrate that mono cyte-derived IL-15 is critical for optimal NK cell production of IFN-g amma, Endogenous IL-15 activates NK cells through the IL-2 receptor, a nd with endogenous IL-12, regulates NK cell IFN-gamma production after monocyte activation by LPS, These in vitro studies are the first to c haracterize a function for endogenous IL-15, and as such, suggest an i mportant role for IL-15 during the innate immune response, IL-15 may b e an important ligand for the NK cell IL-2 receptor in vivo.