EVIDENCE OF APOPTOTIC CELL-DEATH AFTER EXPERIMENTAL TRAUMATIC BRAIN INJURY IN THE RAT

Citation
A. Rink et al., EVIDENCE OF APOPTOTIC CELL-DEATH AFTER EXPERIMENTAL TRAUMATIC BRAIN INJURY IN THE RAT, The American journal of pathology, 147(6), 1995, pp. 1575-1583
Citations number
29
Language
INGLESE
art.tipo
Note
Categorie Soggetti
Pathology
ISSN journal
0002-9440
Volume
147
Issue
6
Year of publication
1995
Pages
1575 - 1583
Database
ISI
SICI code
0002-9440(1995)147:6<1575:EOACAE>2.0.ZU;2-T
Abstract
Apoptosis plays an important role in many developmental and pathologic al processes of the central nervous system. However, the role of apopt osis in traumatic brain injury has not been determined. Using the term inal deoxynucleotidyl transferase-mediated biotinylated deoxyuridine t riphosphate nick end labeling (TUNEL) method, we detected many cells w ith extensive DNA fragmentation in different regions of the brains of rats subjected to experimental traumatic brain injury. Two type of TUN EL-positive cells were demonstrated by light and electron microscopy, including type I cells that displayed morphological feature of necroti c cell death and type II cells that displayed morphological features o f classic apoptotic cell death. TUNEL-positive cells were detectable f or up to 72 hours after the initial injury. Gel electrophoresis of DNA extracted from affected areas of the injured brain containing both ty pe I and II cells revealed only internucleosomal fragmentation at 185- bp intervals, a feature originally described in apoptotic cell death. These data suggest that apoptosis, in addition to necrotic cell death, occurs after traumatic brain injury, and that internucleosomal fragme ntation of DNA may be associated with certain types of necrotic cell d eath.