THE ORPHAN NUCLEAR RECEPTOR TR2 SUPPRESSES A DR4 HORMONE RESPONSE ELEMENT OF THE MOUSE CRABP-I GENE PROMOTER

Citation
C. Chinpaisal et al., THE ORPHAN NUCLEAR RECEPTOR TR2 SUPPRESSES A DR4 HORMONE RESPONSE ELEMENT OF THE MOUSE CRABP-I GENE PROMOTER, Biochemistry, 36(46), 1997, pp. 14088-14095
Citations number
36
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology
Journal title
ISSN journal
0006-2960
Volume
36
Issue
46
Year of publication
1997
Pages
14088 - 14095
Database
ISI
SICI code
0006-2960(1997)36:46<14088:TONRTS>2.0.ZU;2-K
Abstract
The mouse orphan nuclear receptor TR2-11-f suppressed the expression o f reporters fused to a hormone response element of the mouse cellular retinoic acid-binding protein I gene promoter. TR2-11-f was able to bi nd to a direct repeat with four nucleotides in the spacer (5'TGACCTTTG GGGACCT3') located within this hormone response element as homodimers. The specificity of protein-DNA interactions was demonstrated by compe tition in gel retardation and antibody-mediated supershift reactions, The residues critical for TR2-11-f binding were mapped to both repeate d sequences, whereas the spacer and the flanking sequences were less i mportant. The K-d and B-max of TR2-11-f homodimer binding to this dire ct repeat were determined to be 2.6 nM and 0.012 nhl, respectively, By using a yeast two-hybrid system, it was demonstrated that dimerizatio n of TR2-11-f was mediated by its ligand-binding domain, The actions o f TR2-11-f in regulating cellular retinoic acid-binding protein I gene will likely influence retinoic action and availability within the cel ls.