T-CELL EPITOPE MAPPING THE PORB PROTEIN OF SEROGROUP-B NEISSERIA-MENINGITIDIS IN B10 CONGENIC STRAINS OF MICE

Citation
Aa. Delvig et al., T-CELL EPITOPE MAPPING THE PORB PROTEIN OF SEROGROUP-B NEISSERIA-MENINGITIDIS IN B10 CONGENIC STRAINS OF MICE, Clinical immunology and immunopathology, 85(2), 1997, pp. 134-142
Citations number
44
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pathology,Immunology
ISSN journal
0090-1229
Volume
85
Issue
2
Year of publication
1997
Pages
134 - 142
Database
ISI
SICI code
0090-1229(1997)85:2<134:TEMTPP>2.0.ZU;2-N
Abstract
T-cell epitope mapping the meningococcal serotype 15 PorB protein perf ormed in this study in three congenic strains of mice with B10 genetic background revealed at least three murine T-cell epitopes (55-72, 163 -180, and 226-261), located in the highly conserved putative transmemb rane regions of Neisserial porins, Proliferation assays with popliteal lymph node cells derived from mice immunized with the PorB protein or with synthetic 18-mer peptides showed that epitope 163-180 immunized only in the H-2(d) haplotype, epitope 55-72 could be presented by both H-2(f) and H-2(s) molecules, while the 226-261 region covered by thre e overlapping peptides could be efficiently recognized in context of a ll three MHC class II haplotypes studied. Inhibition experiments with blocking I-A alpha- and I-E alpha-specific mAb showed that peptide 163 -180 was presented by I-A(d) and peptide 244-261 was presented by both I-A(f) and I-A(s). In addition, evidence was obtained that peptide 22 6-243 was presented in context of H-2(d) or I-A(s) haplotypes and pept ide 55-72 was presented in context of I-A(f) and I-A(s) loci. Finally, the Norwegian outer membrane vesicle vaccine, but not the purified Po rB protein, could recall responses in mice immunized with synthetic pe ptides corresponding to the 226-261 region. Altogether, these results suggest that T-cell epitopes identified on the serotype 15 PorB protei n, particularly those presented by several MHC class II molecules (e.g ., 226-261), could have important implications for the development of meningococcal vaccines. (C) 1997 Academic Press.