IMPAIRED MOUSE FERTILIZATION BY LOW CHRONIC ALCOHOL TREATMENT

CEBRAL E LASSERRE A RETTORI V DEGIMENO MAF

E. Cebral et al., IMPAIRED MOUSE FERTILIZATION BY LOW CHRONIC ALCOHOL TREATMENT, Alcohol and alcoholism, 32(5), 1997, pp. 563-572

40

INGLESE

Article

Substance Abuse

Alcohol and alcoholism → ACNP

0735-0414

32

5

1997

563 - 572

ISI

0735-0414(1997)32:5<563:IMFBLC>2.0.ZU;2-M

Little is known about the effects of low chronic alcohol intake on fer tility, particularly in females. Recently, we have shown that chronic 10% (w/v) ethanol treatment affects in-vitro fertilization of mouse fe male gamete. The aim of this study was to solve questions concerning t he lowest dose and duration of ethanol treatment required to alter the fertility of immature and adult female and adult male mouse. Mice wer e treated with 5% and 2.5% (w/v) ethanol in drinking water for 4 weeks . The in-vitro fertilization rates were significantly decreased with t he 5% ethanol when oocytes from prepubertal and pubertal ethanol-treat ed females were inseminated with spermatozoa from adult control males. The in-vitro fertilization rates were not diminished when oocytes fro m control females were inseminated with spermatozoa from adult ethanol -treated males. Haploid oocytes were increased when oocytes came from immature females treated with ethanol. The in-vitro fertilization rate s were not decreased in adult treated females. The in-vivo fertilizati on rates were not modified when prepubertal ethanol-treated females we re mated with adult control males. Fragmented oocytes, in the in-vitro fertilization experiments, were significantly increased when they cam e from prepubertal and adult treated females inseminated with ethanol- treated males. These results show that there is a threshold of the eth anol dose to produce an effect. Chronic low ethanol ingestion by immat ure female mice has a deleterious effect on their in-vitro fertilizati on. Furthermore, acute ethanol ingestion by adult females during the i nduction of ovulation resulted in high parthenogenetic activation and fragmentation of mouse oocytes.