OXIDATIVE DNA-BASE DAMAGE AND ANTIOXIDANT ENZYME LEVELS IN CHILDHOOD ACUTE LYMPHOBLASTIC-LEUKEMIA

Citation
S. Senturker et al., OXIDATIVE DNA-BASE DAMAGE AND ANTIOXIDANT ENZYME LEVELS IN CHILDHOOD ACUTE LYMPHOBLASTIC-LEUKEMIA, FEBS letters, 416(3), 1997, pp. 286-290
Citations number
36
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biophysics,Biology
Journal title
ISSN journal
0014-5793
Volume
416
Issue
3
Year of publication
1997
Pages
286 - 290
Database
ISI
SICI code
0014-5793(1997)416:3<286:ODDAAE>2.0.ZU;2-N
Abstract
We have investigated the levels of several antioxidant enzymes and the level of oxidative DNA base damage in lymphocytes of children with ac ute lymphoblastic leukemia (ALL) and in disease-free children, Childre n with ALL had just been diagnosed,vith the disease and had received n o therapy prior to obtaining blood samples, A multitude of typical hyd roxyl radical-induced base lesions in lymphocyte DNA of children were identified and quantified by gas chromatography-isotope dilution mass spectrometry. Higher levels of DNA base lesions were observed in patie nts with ALL than in children without the disease, The levels of the a ntioxidant enzymes glutathione peroxidase, catalase and superoxide dis mutase in lymphocytes of ALL patients were lower than in lymphocytes o f controls, These findings are in agreement with earlier observations in various types of adulthood cancer, Some of the identified DNA base lesions are known to possess premutagenic properties and may play a ro le in carcinogenesis, The results may indicate a possible link between decreased activities of antioxidant enzymes and increased levels of D NA base lesions due to oxidative damage, and support the notion that f ree radical reactions may be increased in malignant cells. (C) 1997 Fe deration of European Biochemical Societies.