OXIDATIVE DNA-BASE DAMAGE AND ANTIOXIDANT ENZYME LEVELS IN CHILDHOOD ACUTE LYMPHOBLASTIC-LEUKEMIA

SENTURKER S KARAHALIL B INAL M YILMAZ H MUSLUMANOGLU H GEDIKOGLU G DIZDAROGLU M

S. Senturker et al., OXIDATIVE DNA-BASE DAMAGE AND ANTIOXIDANT ENZYME LEVELS IN CHILDHOOD ACUTE LYMPHOBLASTIC-LEUKEMIA, FEBS letters, 416(3), 1997, pp. 286-290

36

INGLESE

Article

Biophysics,Biology

FEBS letters → ACNP

0014-5793

416

3

1997

286 - 290

ISI

0014-5793(1997)416:3<286:ODDAAE>2.0.ZU;2-N

We have investigated the levels of several antioxidant enzymes and the level of oxidative DNA base damage in lymphocytes of children with ac ute lymphoblastic leukemia (ALL) and in disease-free children, Childre n with ALL had just been diagnosed,vith the disease and had received n o therapy prior to obtaining blood samples, A multitude of typical hyd roxyl radical-induced base lesions in lymphocyte DNA of children were identified and quantified by gas chromatography-isotope dilution mass spectrometry. Higher levels of DNA base lesions were observed in patie nts with ALL than in children without the disease, The levels of the a ntioxidant enzymes glutathione peroxidase, catalase and superoxide dis mutase in lymphocytes of ALL patients were lower than in lymphocytes o f controls, These findings are in agreement with earlier observations in various types of adulthood cancer, Some of the identified DNA base lesions are known to possess premutagenic properties and may play a ro le in carcinogenesis, The results may indicate a possible link between decreased activities of antioxidant enzymes and increased levels of D NA base lesions due to oxidative damage, and support the notion that f ree radical reactions may be increased in malignant cells. (C) 1997 Fe deration of European Biochemical Societies.