AMPLIFICATION OF THE C-ERBB-2(HER-2 NEU) PROTOONCOGENE IN OVARIAN CARCINOMAS/

Citation
Qb. Fan et al., AMPLIFICATION OF THE C-ERBB-2(HER-2 NEU) PROTOONCOGENE IN OVARIAN CARCINOMAS/, Chinese medical journal, 107(8), 1994, pp. 589-593
Citations number
11
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medicine, General & Internal
Journal title
ISSN journal
0366-6999
Volume
107
Issue
8
Year of publication
1994
Pages
589 - 593
Database
ISI
SICI code
0366-6999(1994)107:8<589:AOTCNP>2.0.ZU;2-#
Abstract
C-erbB-2(HER-2 / neu) proto-oncogene is mainly expressed in epithelial tissue and activated due to its amplification. Amplification of the C -erbB-2 proto-oncogene has been associated with poor prognosis in huma n ovarian cancer. Our study was to examine whether amplification is mo re frequently observed in ovarian cancer, or it is associated with poo r prognosis of human ovarian cancer in China. The DNA of ovarian cance rs was extracted and consequently digested with restriction endonuclea se EcoRI, electrophoresed in 0.8% agarose gels and blotted onto nitroc ellulose filter with Southern transfering method. It was then hybridiz ed with a P-32-labelled C-erbB-2 probe and subsequently underwent auto radiography. The result has shown that the C-erbB-2(HER-2 / neu) gene was amplified in 8 of 26 human ovarian cancers (30.8%). The clinical d ata showed that all of the 8 cases with the amplified C-erbB-2 were in their advanced stage (III-IV). Five of the patients died from 2 to 4 months after operation. These data suggest that amplification of the C -erbB-2 gene may play a role in the pathogenesis of ovarian carcinoma; it is frequently observed in advanced ovarian cancer and is associate d with poor prognosis for these patients.