APOLIPOPROTEIN(A) INDUCES MONOCYTE CHEMOTACTIC ACTIVITY IN HUMAN VASCULAR ENDOTHELIAL-CELLS

Citation
M. Poon et al., APOLIPOPROTEIN(A) INDUCES MONOCYTE CHEMOTACTIC ACTIVITY IN HUMAN VASCULAR ENDOTHELIAL-CELLS, Circulation, 96(8), 1997, pp. 2514-2519
Citations number
44
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Peripheal Vascular Diseas",Hematology
Journal title
ISSN journal
0009-7322
Volume
96
Issue
8
Year of publication
1997
Pages
2514 - 2519
Database
ISI
SICI code
0009-7322(1997)96:8<2514:AIMCAI>2.0.ZU;2-P
Abstract
Background Elevated levels of lipoprotein(a) [Lp(a)] are associated wi th premature atherosclerosis; however, the mechanisms are not known. R ecruitment of monocytes to the blood vessel wall is an early event in atherogenesis. Methods and Results This study has found that unoxidize d Lp(a) induced human umbilical vein endothelial cells (HUVECs) to sec rete monocyte chemotactic activity (MCA), whereas LDL under the same c onditions did not. In the absence of HUVECs, Lp(a) had no direct MCA. Endotoxin was shown not to be responsible for the induction of MCA. Ac tinomycin D and cycloheximide inhibited the HUVEC response to Lp(a), i ndicating that protein and RNA synthesis were required. The apolipopro tein(a) [apo(a)] portion of Lp(a) was identified as the structural com ponent of Lp(a) responsible for inducing MCA. Lp(a) and apo(a) also st imulated human coronary artery endothelial cells to produce MCA. Granu locyte-monocyte colony-stimulating factor (GM-CSF) antigen was not det ected in the Lp(a)-conditioned medium, nor was monocyte chemoattractan t protein-1 mRNA induced in HUVECs by Lp(a). Conclusions These finding s suggest that Lp(a) may be involved in the recruitment of monocytes t o the vessel wall and provide a novel mechanism for the participation of Lp(a) in the atherogenic process.