AMINO-TERMINUS TRUNCATED APOLIPOPROTEIN-E IS THE MAJOR SPECIES IN AMYLOID DEPOSITS IN ALZHEIMERS-DISEASE AFFECTED BRAINS - A POSSIBLE ROLE FOR APOLIPOPROTEIN-E IN ALZHEIMERS-DISEASE

Citation
Y. Aizawa et al., AMINO-TERMINUS TRUNCATED APOLIPOPROTEIN-E IS THE MAJOR SPECIES IN AMYLOID DEPOSITS IN ALZHEIMERS-DISEASE AFFECTED BRAINS - A POSSIBLE ROLE FOR APOLIPOPROTEIN-E IN ALZHEIMERS-DISEASE, Brain research, 768(1-2), 1997, pp. 208-214
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
0006-8993
Volume
768
Issue
1-2
Year of publication
1997
Pages
208 - 214
Database
ISI
SICI code
0006-8993(1997)768:1-2<208:ATAITM>2.0.ZU;2-X
Abstract
Amyloid deposits in Alzheimer's disease (AD) are composed of amyloid b eta protein (A beta) and many other components called amyloid-associat ed proteins. Apolipoprotein E (apoE) is one of the most important amyl oid-associated proteins. The role apoE plays in AD, however, is yet to be determined. Zn this study, we present the biochemical and histoche mical nature of apoE in AD-affected brains using four monoclonal antib odies (mAbs) against apoE and newly established antibodies against the amino-terminal (anti-apoE-N), and carboxyl-terminal regions (anti-apo E-C) of apoE. Competitive ELISA and Western-blot analysis combined wit h thrombolytic digestion of apoE indicated that our four mAbs recogniz ed at least two different epitopes within a 22-kDa amino-terminal doma in of apoE. Using these mAbs and an anti-A beta mAb, double immunostai ning showed that the majority of amyloid deposits were stained by both anti-apoE and anti-AP mAbs, but the minority of them were detected on ly by either anti-apoE or anti-A beta mAbs. Differences in staining pr operties between anti-apoE-N and anti-apoE-C were that anti-apoE-C rec ognized both amyloid deposits and astrocytes similar to anti-apoE mAbs , but anti-apoE-N strongly stained only astrocytes. Preliminary semi-q uantitative determinations of apoE in CSF and brain homogenate showed that the amount of apoE increased in AD and Creutzfeldt-Jakob disease brains compared to normal samples. Our immunological data, using antib odies specific for the amino and carboxyl termini of apoE, suggest tha t apoE may, in some circumstances, initiate plaque formation, and that apoE in amyloid deposits has at least part of its amino termini cleav ed out. (C) 1997 Elsevier Science B.V.