ION-MOLECULE REACTIONS AS PROBES OF GAS-PHASE STRUCTURES OF PEPTIDES AND PROTEINS

Citation
Mk. Green et Cb. Lebrilla, ION-MOLECULE REACTIONS AS PROBES OF GAS-PHASE STRUCTURES OF PEPTIDES AND PROTEINS, Mass spectrometry reviews, 16(2), 1997, pp. 53-71
Citations number
116
Language
INGLESE
art.tipo
Review
Categorie Soggetti
Spectroscopy
Journal title
ISSN journal
0277-7037
Volume
16
Issue
2
Year of publication
1997
Pages
53 - 71
Database
ISI
SICI code
0277-7037(1997)16:2<53:IRAPOG>2.0.ZU;2-R
Abstract
A review with over 100 references describes the recent applications of ion-molecule reactions to the study of gas-phase protonated peptides and proteins. The topic is focused specifically on the proton transfer and hydrogen-deuterium exchange reactions of amino acids, peptides, a nd proteins. A brief background is given of the various methods used f or assigning proton affinities and gas-phase basicities. The methods u sed for measuring the kinetics of deuterium incorporation of charged i on in the presence of a background pressure of deuterating reagents ar e also described. Ion-molecule reactions are used to determine, among other things, the gas-phase basicities and proton affinities of amino acids, peptides, and proteins, the sites of protonation, intra- and in termolecular interactions, and conformational differences and changes in gas-phase ionic species. Singly charged and multiply charged ions a re both covered. (C) 1997 John Wiley & Sons, Inc.